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| Registros recuperados: 22 | |
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| DRESCH,DAIANE M.; MASETTO,TATHIANA E.; SCALON,SILVANA P.Q.. |
| The aim of this study was to evaluate the sensitivity of Campomanesia adamantium seeds to desiccation by drying in activated silica gel (fast) and under laboratory conditions (slow). To assess the sensitivity of the seeds to desiccation, we used drying with silica gel and drying under laboratory conditions (25 °C), in order to obtain seeds with moisture content of 45, 35, 30, 25, 20, 15, 10 and 5%. The physiological potential of the seeds after desiccation was evaluated by measuring primary root protrusion, percentage of normal seedlings, germination seed index, seedling length, total seedling dry mass, electrical conductivity and DNA and RNA integrities. The C. adamantium seeds were sensitive to desiccation and to a reduction in moisture content to 21.1%... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cerrado; DNA; Drying; RNA; Viability. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000502217 |
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| García-Nogales,Paula; Serrano,Alicia; Secchi,Sonia; Gutiérrez,Serafín; Arís,Anna. |
| Nucleic-acid based methods for bacterial identification are extremely useful in diagnostic applications due to their specificity and sensitivity. However, they require an optimal purification of the target molecules. As part of the development of a new diagnostic method for the detection of bacterial RNA in cow milk, we have compared four commercially available RNA extraction kits for the isolation of bacterial RNA from spiked UHT milk samples. The kits were compared in terms of extraction efficiency and RNA purity using two bacterial species, the Gram negative Escherichia coli and the Gram positive Staphylococcus aureus. Two kits are based in silica-matrix extraction, and the other two in the guanidinium thiocyanate-phenol-chloroform extraction. In our... |
| Tipo: Journal article |
Palavras-chave: Bacteria; Milk; Purification; RNA. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500019 |
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| Teixeira,S.M.R.. |
| The study of mechanisms which control gene expression in trypanosomatids has developed at an increasing rate since 1989 when the first successful DNA transfection experiments were reported. Using primarily Trypanosoma brucei as a model, several groups have begun to elucidate the basic control mechanisms and to define the cellular factors involved in mRNA transcription, processing and translation in these parasites. This review focuses on the most recent studies regarding a subset of genes that are expressed differentially during the life cycle of three groups of parasites. In addition to T. brucei, I will address studies on gene regulation in a few species of Leishmania and the results obtained by a much more limited group of laboratories studying gene... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Trypanosoma; Leishmania; Gene expression; RNA; Antigenic variation; VSG. |
| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001200001 |
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| CORDEIRO, M. C. R.. |
| ABSTRACT: Basic concepts and methodologies concerning genetic engineering are presented in this review and is directed to people or professionales not familiarized to it. Moreover, it can be a document to clarity what is genetic engineering, the importance of its contributions to modern agriculture, medicine or livestock research and to support discussions on genetic research ethics. Covers information on structure of nucleic acids, types, functions and main processes such us DNA duplication, transcription and traduction. Important methodologies are covered about DNA/RNA extraction, electrophoresis, PCR, nucleic acid libraries production, cloning, sequencing, gene characterization in connection to main historical events of genetic engineering. It... |
| Tipo: Documentos (INFOTECA-E) |
Palavras-chave: Ácido Nucléico; Biologia Molecular; Biotecnologia; Clonagem; DNA; Engenharia Genética; Gene; Marcador Genético; RNA; Organismo Transgênico.. |
| Ano: 2003 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/568132 |
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| Maitre, J; Mercier, L; Dolo, L; Valotaire, Y. |
| In the liver of rainbow trout (Salmo gairdnerii ), vitellogenin (Vg) synthesis is influenced by oestradiol (E sub(2)) which is believed to act through the classical mechanism of steroid hormone action. After binding of the hormone to a soluble specific receptor protein, the oestradiol-receptor complex is translocated to the nucleus, where it interacts with DNA and modulates expression of Vg genes, leading to increased synthesis of specific mRNA and Vg. The authors show here: (i) the presence of specific oestrogen receptors in the cytosol of the male trout liver. (ii) The male liver, offering, an ideal experimental control of "zero" background, the authors followed - in the liver of male trouts - the kinetics of induction of Vg mRNA by hybridization with Vg... |
| Tipo: Text |
Palavras-chave: Salmonidae; Pisces; Proteins; Liver; RNA; Steroids; Biosynthesis; Receptors; Sex hormones; Sexual reproduction. |
| Ano: 1983 |
URL: http://archimer.ifremer.fr/doc/1983/acte-1230.pdf |
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| Barragan,Carlos Eduardo; Guzmán-Barney,Mónica. |
| Potato yellow vein virus (PYVV) expresses yellowing symptoms on plants and it is transmitted by the whitefly vector Trialeurodes vaporariorum Westwood. No molecular viral detection from the vector extracts has been reported to date. The objectives of this study were I) amplification of the PYVV major coat protein (CP) gene from viruliferous vector extracts by RT-PCR using specific primers, II) cloning and III) sequencing and sequences analysis. Some Colombian Solanum phureja (Jus et Buk) field potato plants expressing yellowing symptoms and confirmed PYVV positive by RT-PCR were used as virus donor plants for feeding whiteflies. Viruliferous vectors were obtained by depositing aviruliferous whiteflies on symptomatic leaves and kept in a muslin... |
| Tipo: Journal article |
Palavras-chave: PYVV; RNA; Whitefly; Detection; Vector. |
| Ano: 2014 |
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1010-27522014000300002 |
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| Oliva Teles, A; Guedes, M; Vachot, Christianne; Kaushik, Sadasivam. |
| The aim of this trial was to evaluate the effect of dietary nucleic acids on ureagenesis and nitrogen balance in gilthead sea bream juveniles. For that purpose, 5 isonitrogenous (7.25% N) diets were formulated based on fish meal as protein source (FM-control diet) and to partially replace the fish meal nitrogen with two levels of nucleic acids (diets RNA1 and RNA2) or brewers yeast (diets BY1 and BY2). Each diet was fed for 10 weeks, to apparent visual satiety, to triplicate groups of 50 fish each with an average body weight of 12.7 g. Inclusion of nucleic acid N either as brewers yeast or RNA led to significant improvement of feed intake and growth, except for fish fed diet RNA2. There were no differences in final weight between groups fed the yeast-... |
| Tipo: Text |
Palavras-chave: Sea bream; N accretion; Ureagenesis; Brewers yeast; RNA. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-6681.pdf |
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| Registros recuperados: 22 | |
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